Journal: Frontiers in Immunology
Article Title: Single-cell RNA sequencing unraveled immune-related expression heterogeneity and lymphoid cell development dysregulation in childhood asthma
doi: 10.3389/fimmu.2025.1606650
Figure Lengend Snippet: Cell communication pattern for asthma patients and healthy controls, serum resistin expression level and its PPI network. (A) The number of cell-cell interactions and interaction strength for four sample groups. (B) The annexin signaling pathway network of three sample groups. The annexin signaling pathway network in all cell types is shown for healthy controls, Asthma 1 and Asthma 3. The line thickness represents the signal strength, e.g. CD14 + monocytes have an annexin strength of 0.00006 in control, 0.0009 in asthma 1, and 0.0007 in asthma 3, respectively. The signal strength was estimated with the gene expression data of ligand-receptor pair. (C) The resistin signaling pathway network of three sample groups. The resistin signaling pathway network in all cell types is shown for healthy controls, Asthma 2 and Asthma 3. The line thickness represents the signal strength, e.g. CD14 + monocytes have a resistin strength of 0.0005 in control, 0.0025 in asthma 2, and 0.006 in asthma 3, respectively. The signal strength was estimated with the gene expression data of ligand-receptor pair. (D) The expression of RETN gene in two sample groups. The level of serum resistin between asthma patients and healthy controls (*** p < 0.001). (E) Protein–protein interaction network of RETN, its directly interacting genes and their neighbors. RETN is marked with red color and SQSTM1, HSPA5, and A2M are marked with light green color. RETN has a network degree of 8. SQSTM1, HSPA5, and A2M have a network degree of 109, 44, and 37, respectively. Network degree represents the number of neighbors (genes) connected to a hub, which is a core metric for measuring node importance and connection strength in complex network analysis.
Article Snippet: We followed a human resistin ELISA kit (Signalway Antibody, Maryland, USA, Catalog No: EK2351) protocol to analyze peripheral blood serums.
Techniques: Expressing, Control, Gene Expression